Populated places established in 1540

Arequipa (; and ), also known by its nicknames of Ciudad Blanca (Spanish for "White City") and León del Sur (Spanish for "Lion of the South"), is a city in Peru and the capital of the eponymous province and department. It is the seat of the Constitutional Court of Peru and often dubbed the "legal capital of Peru". It is the second most populated city in Peru, after the capital Lima, with an urban population of 1,195,700 in 2025. Known for its colonial architecture and volcanic stone buildings, it is a major cultural and economic center.

Ternopil, known until 1944 mostly as Tarnopol, is a city in western Ukraine, located on the banks of the Seret River. Ternopil is one of the major cities of Western Ukraine and the historical regions of Galicia and Podolia. The population of Ternopil was estimated at

capital city of the Mexican state of Campeche

Jamnagar () is a city and the headquarters of Jamnagar district in the Indian state of Gujarat. The city lies just to the south of the Gulf of Kutch, some west of the state capital, Gandhinagar. The city was the capital of Nawanagar princely state during British Raj.

Ayacucho (, , derived from the words aya ("death" or "soul") and ''k'uchu'' ("corner") in honour of the battle of Ayacucho), founded in 1540 as San Juan de la Frontera de Huamanga and known simply as Huamanga (Quechua: Wamanga) until 1825, is the capital city of Ayacucho Region and of Huamanga Province, Ayacucho Region, Peru.

Abancay (from Quechua language: Hamanqay, Amanqay, or Amankay, meaning lily), founded in 1572 as Santiago de los Reyes de Abancay, is a city in southern-central Peru. It is the capital of both the Apurímac Region and the Abancay Province, and serves an important cultural, economic, and political role in Apurímac.

Moyobamba () or Muyupampa (Quechua muyu circle, pampa large plain, "circle plain"), also called "Santiago of eight valleys of Moyobamba" or "Maynas capital", is the capital city of the San Martín Region in northern Peru. There are 50,073 inhabitants, according to the 2017 census. Some 3,500 species of orchids are native to the area, which has led to another of the city's nicknames , The City of Orchids. The city is the capital of both Moyobamba Province and Moyobamba District.

Colombian municipality of the department of Valle del Cauca

town in the Czech Republic

Oiba () is a town and municipality in the Santander Department in northeastern Colombia. Its antipode Oiba borders Guapotá and Confines in the north; Charalá in the east; Guadalupe and Guapotá in the west; and Suaita in the south. The department capital Bucaramanga is to the north and the national capital Bogotá to the south.

Charalá is a town and municipality in the south of the department of Santander in northeastern Colombia. Its antipode is located within the capital of Indonesia, Jakarta.

municipality in the State of Guanajuato, Mexico

village in Zviahel Raion, Zhytomyr Oblast, Ukraine

municipal seat of San Martín Hidalgo, State of Jalisco, Mexico

Gargaon (Tai-Ahom:Tsé-hung;) is a historic city in Assam, India and served as the capital of the Ahom kingdom for many years. It was built by the Ahom king Suklenmung (Gargaiya Roja) in 1540. It lies 13 km east of present-day Sivasagar City, headquarters of the Sivasagar district. The palace structures were made of stone and wood. In 1747, Pramatta Singha, son of Rudra Singha, constructed a brick wall of about 5 km in length surrounding the Gargaon palace and the masonry gate leading to it to boost security.

town in San Martín Hidalgo Municipality, State of Jalisco, Mexico