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PIPES
Sign in to saveAlso known as piperazine-N,N′-bis(2-ethanesulfonic acid)
PIPES ('piperazine-N,N-bis(2-ethanesulfonic acid)') is a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.
In the Vinony graph
Vinony's link graph records 8 inbound references to PIPES, and connects out to buffer solution, acid dissociation constant and 1-phenylpiperazine.
It is catalogued under topics including Buffer solutions, ECHA InfoCard ID from Wikidata and Piperazines.
Vinony links it to 7 Wikipedia language editions.
Chemical data
- Formula
- C8H18N2O6S2
- Molecular weight
- 302.4 g/mol
- IUPAC name
- 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
- SMILES
- C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
- InChIKey
- IHPYMWDTONKSCO-UHFFFAOYSA-N
- XLogP
- -6.7
- Polar surface area
- 132 Ų
- H-bond donors
- 2
- H-bond acceptors
- 8
- Formal charge
- 0
via PubChem
Wikidata facts
- Subclass of
- chemical compound
- Mass
- 302.061
- Has use
- buffering agent
Show 2 more facts
- canonical SMILES
- C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
- chemical formula
- C₈H₁₈N₂O₆S₂
Sources (2)
via Wikidata · CC0
~1 min read
Encyclopedic overview
3 sectionsContents
- Applications
- See also
- References
PIPES ('piperazine-N,N-bis(2-ethanesulfonic acid)') is a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.
==Applications== PIPES has two pKa values. One pKa (6.76 at 25 °C) is near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25 °C. The second pKa value is at 2.67 with a buffer range of from 1.5-3.5. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer. It has a negligible capacity to bind divalent ions.
Excerpted from Wikipedia’s “PIPES” article, available under the CC BY-SA 4.0 licence.