PIPES
Sign in to saveAlso known as piperazine-N,N′-bis(2-ethanesulfonic acid)
PIPES ('piperazine-N,N-bis(2-ethanesulfonic acid)') is a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.
Wikidata facts
- Mass
- 302.061
Show 2 more facts
- canonical SMILES
- C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
- chemical formula
- C₈H₁₈N₂O₆S₂
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3 sectionsContents
- Applications
- See also
- References
PIPES ('piperazine-N,N-bis(2-ethanesulfonic acid)') is a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.
==Applications== PIPES has two pKa values. One pKa (6.76 at 25 °C) is near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25 °C. The second pKa value is at 2.67 with a buffer range of from 1.5-3.5. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer. It has a negligible capacity to bind divalent ions.