3,3',5,5'-tetramethylbenzidine
Also known as TMB
3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). TMB is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights. Used to detect hematuria as it turns blue in contact with hemoglobin.
Research
2,774 papers- 3,3',5,5'-Tetramethylbenzidine Can Be Unsuitable for Identifying Peroxidases.ACS sensors · 2024
- Oxidized 3,3',5,5'-tetramethylbenzidine nanobelts enhance colorimetric sensing of H(2)O(2).Talanta · 2024
- Rationale of 3,3',5,5'-Tetramethylbenzidine as the Chromogenic Substrate in Colorimetric Analysis.Analytical chemistry · 2020
- Advantages and limitations of 3,3',5,5'-tetramethylbenzidine for immunohistochemical staining.Histology and histopathology · 2025
- Bioenzyme-free colorimetric assay for creatinine determination based on Mn(3)O(4) nanoparticles catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine.Mikrochimica acta · 2023
via PubMed
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Article
3 sectionsContents
- Enzymatic assay
- Material safety
- References
3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). TMB is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights. Used to detect hematuria as it turns blue in contact with hemoglobin.
==Enzymatic assay==