File:ELISA_TMB.jpg · Wikimedia Commons · See Wikimedia Commons
ELISA
Sign in to saveAlso known as enzyme-linked immunosorbent assay, enzyme-linked immunosorbant assay, EIA
The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay is a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the ligand to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries.
Key facts
- Interventions.Name
- ELISA
- Interventions.Image
- ELISA TMB.jpg
- Interventions.Caption
- An ELISA being developed with TMB substrate for horseradish peroxidase-linked secondary antibody
- Interventions.MeshID
- D004797
via Wikipedia infobox
Research
366,211 papersvia PubMed
Wikidata facts
- Image
- Microtiter plate.JPG
Show 2 more facts
- Commons category
- Enzyme-linked immunosorbent assay
- short name
- ІФА
Sources (2)
via Wikidata · CC0
~23 min read
Article
16 sectionsContents
- Principle
- History
- Types
- Direct
- Sandwich
- Competitive
- Indirect
- Commonly used enzymatic markers
- Applications
- Enzyme-Linked Single Molecule Array (eSimoa)
- Technology
- Applications
- Origins and Controversy
- See also
- Notes and references
- External links
The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay is a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the ligand to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries.
In the most simple form of an ELISA, antigens from the sample to be tested are attached to a surface. Then, a matching antibody is applied over the surface so it can bind the antigen. This antibody is linked to an enzyme, and then any unbound antibodies are removed. In the final step, a substance containing the enzyme's substrate is added. If there was binding, the subsequent reaction produces a detectable signal, most commonly a color change.