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immunostaining

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thumb|350px|Micrograph of a GFAP immunostained section of a [[brain tumour.]]

~8 min read

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10 sections
Contents
  • Techniques
  • Immunohistochemistry
  • Flow cytometry
  • Western blotting
  • Enzyme-linked immunosorbent assay
  • Immuno-electron microscopy
  • Methodological overview
  • Applications
  • See also
  • References

thumb|350px|Micrograph of a GFAP immunostained section of a [[brain tumour.]]

In biochemistry, immunostaining is any use of an antibody-based method to detect a specific protein in a sample. The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. However, immunostaining now encompasses a broad range of techniques used in histology, cell biology, and molecular biology that use antibody-based staining methods.

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