aequorin
Sign in to saveAequorin is a calcium-activated photoprotein isolated from the hydrozoan Aequorea victoria. Its bioluminescence was studied decades before the protein was isolated from the animal by Osamu Shimomura in 1962. In the animal, the protein occurs together with the green fluorescent protein to produce green light by resonant energy transfer, while aequorin by itself generates blue light.
Key facts
- Nonhuman protein.Name
- Aequorin 1
- Nonhuman protein.image
- Aequorin 1EJ3.png
- Nonhuman protein.caption
- Aequorin ribbon diagram from PDB 1ej3 with prosthetic group coelenterazine in blue
- Nonhuman protein.Organism
- Aequorea victoria (Jellyfish)
- Nonhuman protein.TaxID
- 6100
- Nonhuman protein.Symbol
- N/A
- Nonhuman protein.UniProt
- P07164
- Nonhuman protein.ECnumber
- 1.13.12.5
via Wikipedia infobox
Research
2,022 papers- Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea.Journal of cellular and comparative physiology · 1962
- Using aequorin probes to measure Ca(2+) in intracellular organelles.Cell calcium · 2017
- Recombinant aequorin methods for intracellular calcium measurement in plants.Methods in cell biology · 1995
- Regulation of myocardial contraction as revealed by intracellular Ca(2+) measurements using aequorin.The journal of physiological sciences : JPS · 2024
- Shedding Light on Calcium Dynamics in the Budding Yeast: A Review on Calcium Monitoring with Recombinant Aequorin.Molecules (Basel, Switzerland) · 2024
via PubMed
~11 min read
Encyclopedic overview
8 sectionsContents
- Discovery
- Structure
- Genetics
- Mechanism of action
- Applications
- Marketing and legal challenges
- References
- External links
Aequorin is a calcium-activated photoprotein isolated from the hydrozoan Aequorea victoria. Its bioluminescence was studied decades before the protein was isolated from the animal by Osamu Shimomura in 1962. In the animal, the protein occurs together with the green fluorescent protein to produce green light by resonant energy transfer, while aequorin by itself generates blue light.
Discussions of "jellyfish DNA" that can make "glowing" animals often refer to transgenic animals that express the green fluorescent protein, not aequorin, although both originally derive from the same animal.
Excerpted from Wikipedia’s “aequorin” article, available under the CC BY-SA 4.0 licence.