EcoRI
Sign in to saveAlso known as Restriction endonuclease, type II, EcoRI, Restrct_endonuc_II_EcoRI, IPR004221
EcoRI (pronounced "eco R one") is a type II restriction enzyme isolated from Escherichia coli. It cleaves DNA double helices into fragments at specific sites, and is also a part of the restriction modification system. The enzyme's name originates from the species from which it was isolated: "E" denotes generic name (Escherichia), "co" denotes species name (coli), "R" represents the strain (RY13), and the "I" denotes that it was the first enzyme isolated from this strain.
Key facts
- Protein family.Symbol
- EcoRI
- Protein family.Name
- EcoRI
- Protein family.image
- Ecor1_2ckq.png
- Protein family.caption
- EcoRI crystal structure. Dimer bound to DNA (PDB
- Protein family.Pfam
- PF02963
- Protein family.InterPro
- IPR004221
- Protein family.SCOP
- 1na6
- Protein family.CDD
- 79lll
via Wikipedia infobox
Research
8,897 papers- How the EcoRI endonuclease recognizes and cleaves DNA.BioEssays : news and reviews in molecular, cellular and developmental biology · 1992
- In-silicon studies on hydration in EcoRI-cognate DNA complex.Biophysical chemistry · 2023
- Effect of osmolytes on the EcoRI endonuclease: Insights into hydration and protein dynamics from molecular dynamics simulations.Computational biology and chemistry · 2023
- HindIII/EcoRI polymorphism in the GAA gene.Nucleic acids research · 1990
- Substrate recognition by the EcoRI endonuclease.Proteins · 1990
via PubMed
Wikidata facts
Show 4 more facts
- recognition sequence
- GAATTC
- cutting site of restriction enzyme
- G^AATTC
- produces cohesive end
- ^AATT
- Commons category
- Restriction endonuclease EcoRI
Sources (9)
via Wikidata · CC0
~3 min read
Article
8 sectionsContents
- History
- Structure
- Primary structure
- Tertiary and quaternary structure
- Uses
- See also
- References
- External links
EcoRI (pronounced "eco R one") is a type II restriction enzyme isolated from Escherichia coli. It cleaves DNA double helices into fragments at specific sites, and is also a part of the restriction modification system. The enzyme's name originates from the species from which it was isolated: "E" denotes generic name (Escherichia), "co" denotes species name (coli), "R" represents the strain (RY13), and the "I" denotes that it was the first enzyme isolated from this strain.
In molecular biology it is used for restriction digests. EcoRI creates sticky ends with 5' end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G↓AATTC, which has a palindromic complementary sequence of CTTAA↓G.