HindIII
Sign in to saveAlso known as deoxyribonuclease HindIII
HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis. thumb|HindIII restrictions process results in formation of overhanging palindromic sticky ends. The cleavage of this sequence between the AA's results in 5' overhangs on the DNA called sticky ends:
Key facts
- Protein family.Symbol
- RE_Hindiii
- Protein family.Name
- HindIII restriction endonuclease
- Protein family.image
- 2e52.png
- Protein family.caption
- Crystallographic structure of the HindIII restriction endonuclease dimer (cyan and green) complexed with double helical DNA (brown) based on the coordinates.
- Protein family.Pfam
- PF09518
- Protein family.InterPro
- IPR019043
- Protein.Name
- hindIIIR type II restriction endonuclease
- Protein.Symbol
- hindIIIR
- Protein.EntrezGene
- 950303
- Protein.UniProt
- P43870
- Protein.PDB
- 2e52
- Protein.PDB_supplemental
- More structures
- Protein.ECnumber
- 3.1.21.4
via Wikipedia infobox
Research
5,569 papers- The HindIII and PvuII polymorphisms of lipoprotein lipase (LPL) gene reduce the risk of ischemic stroke (IS): A meta-analysis.Medicine · 2018
- Dyslipidemia: Genetics, lipoprotein lipase and HindIII polymorphism.F1000Research · 2017
- HindIII polymorphism in the BCL6 gene.Human molecular genetics · 1993
- HindIII/EcoRI polymorphism in the GAA gene.Nucleic acids research · 1990
- HindIII polymorphism in the lipoprotein lipase gene and hypertensive intracerebral hemorrhage in the Chinese Han population.Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association · 2014
via PubMed
Wikidata facts
Show 3 more facts
- recognition sequence
- AAGCTT
- cutting site of restriction enzyme
- A^AGCTT
- produces cohesive end
- ^AGCT
via Wikidata · CC0
~6 min read
Article
5 sectionsContents
- Structure
- Site-directed mutagenesis
- Proposed mechanism
- Uses in research
- References
HindIII (pronounced "Hin D Three") is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis. thumb|HindIII restrictions process results in formation of overhanging palindromic sticky ends. The cleavage of this sequence between the AA's results in 5' overhangs on the DNA called sticky ends:
5'-A |A G C T T-3'