ficoll
Sign in to savethumb|upright=2|Components of Ficoll Ficoll is a neutral, highly branched, high-mass, hydrophilic polysaccharide synthesized from repeating sucrose units, which dissolves readily in aqueous solutions. Ficoll radii range from 2 to 7 nm. It is prepared by reaction of the polysaccharide with epichlorohydrin. Ficoll is a registered trademark owned by GE Healthcare companies.
Research
5,536 papers- Ficoll and dextran vs. globular proteins as probes for testing glomerular permselectivity: effects of molecular size, shape, charge, and deformability.American journal of physiology. Renal physiology · 2005
- Ficoll as testing material for diffusion weighted imaging-quality assurance phantoms.Magnetic resonance imaging · 2021
- Contrasting effect of ficoll on apo and holo forms of bacterial chemotaxis protein Y: Selective destabilization of the conformationally altered holo form.International journal of biological macromolecules · 2023
- Ficoll is not a rigid sphere.American journal of physiology. Renal physiology · 2007
- Effects of anticoagulants and Ficoll on human serum antibody reactivities and functions against Mycobacterium tuberculosis.Tuberculosis (Edinburgh, Scotland) · 2020
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thumb|upright=2|Components of Ficoll Ficoll is a neutral, highly branched, high-mass, hydrophilic polysaccharide synthesized from repeating sucrose units, which dissolves readily in aqueous solutions. Ficoll radii range from 2 to 7 nm. It is prepared by reaction of the polysaccharide with epichlorohydrin. Ficoll is a registered trademark owned by GE Healthcare companies.
Ficoll is part of Ficoll-Paque, which is used in biology laboratories to separate blood to its components (erythrocytes, leukocytes etc.). Ficoll-Paque is normally placed at the bottom of a conical tube, and blood is then slowly layered above Ficoll-Paque. After being centrifuged, the following layers will be visible in the conical tube, from top to bottom: plasma and other constituents, a layer of mono-nuclear cells called buffy coat (PBMC/MNC), Ficoll-Paque, and erythrocytes and granulocytes, which should be present in pellet form. This separation allows easy harvest of PBMCs. Note that some red blood cell trapping (presence of erythrocytes and granulocytes) may occur in the PBMC or Ficoll-Paque layer. Major blood clotting may sometimes occur in the PBMC layer. Ethylene diamine tetra-acetate (EDTA) and heparin are commonly used in conjunction with Ficoll-Paque(TM) to prevent clotting and is used as a mononuclear cell separator.