fura-2
Sign in to saveFura-2, an aminopolycarboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium. It was the first widely used dye for calcium imaging, and remains very popular. Fura-2 is excited at 340 nm and 380 nm of light, and the ratio of the emissions at those wavelengths is directly related to the amount of intracellular calcium. Regardless of the presence of calcium, Fura-2 emits at 510 nm of light. The use of the ratio automatically cancels out confounding variables, such as variable dye concentration and cell thickness, making Fura-2 one of the most appr
Research
12,345 papers- Inhibition of Fura-2 sequestration and secretion with organic anion transport blockers.Cell calcium · 1990
- Fura-10, unlike fura-2, is suitable for long-term calcium imaging in natural killer (NK) cells without compromising cytotoxicity and can be combined with target cell death analysis.Cell calcium · 2026
- Mt-fura-2, a Ratiometric Mitochondria-Targeted Ca(2+) Sensor. Determination of Spectroscopic Properties and Ca(2+) Imaging Assays.Methods in molecular biology (Clifton, N.J.) · 2021
- Permeation of topically applied Magnesium ions through human skin is facilitated by hair follicles.Magnesium research · 2016
- Calcium Imaging of Store-Operated Calcium (Ca(2+)) Entry (SOCE) in HEK293 Cells Using Fura-2.Methods in molecular biology (Clifton, N.J.) · 2019
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Wikidata facts
- Mass
- 641.149
Show 4 more facts
- chemical formula
- C₂₉H₂₇N₃O₁₄
- canonical SMILES
- CC1=CC(=C(C=C1)N(CC(=O)O)CC(=O)O)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)O)N(CC(=O)O)CC(=O)O
- subject has role
- fluorophore
- Commons category
- Fura-2
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Encyclopedic overview
2 sectionsContents
- See also
- References
Fura-2, an aminopolycarboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium. It was the first widely used dye for calcium imaging, and remains very popular. Fura-2 is excited at 340 nm and 380 nm of light, and the ratio of the emissions at those wavelengths is directly related to the amount of intracellular calcium. Regardless of the presence of calcium, Fura-2 emits at 510 nm of light. The use of the ratio automatically cancels out confounding variables, such as variable dye concentration and cell thickness, making Fura-2 one of the most appreciated tools to quantify calcium levels. The high photon yield of fura-2 allowed the first real time (video rate) measurements of calcium inside living cells in 1986. More recently, genetically encoded calcium indicators based on spectral variants of the green fluorescent protein, such as Cameleons, have supplemented the use of Fura-2 and other small molecule dyes for calcium imaging, but Fura-2 remains faster.
==See also== Fura-2AM, a membrane-permeant derivative of Fura-2 Indo-1
Excerpted from Wikipedia’s “fura-2” article, available under the CC BY-SA 4.0 licence.