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basic red 2
Sign in to saveAlso known as Safranin
Safranin (Safranin O or basic red 2) is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring cell nuclei red. This is the classic counterstain in both Gram stains and endospore staining. It can also be used for the detection of cartilage, mucin and mast cell granules.
Chemical data
- Formula
- C20H19ClN4
- Molecular weight
- 350.8 g/mol
- IUPAC name
- 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine chloride
- SMILES
- CC1=CC2=C(C=C1N)[N+](=C3C=C(C(=CC3=N2)C)N)C4=CC=CC=C4.[Cl-]
- InChIKey
- OARRHUQTFTUEOS-UHFFFAOYSA-N
- Polar surface area
- 68.8 Ų
- H-bond donors
- 2
- H-bond acceptors
- 4
- Formal charge
- 0
via PubChem
Research
509 papers- Relation between mitochondrial membrane potential and ROS formation.Methods in molecular biology (Clifton, N.J.) · 2012
- Basic red 2 and methyl violet adsorption by date pits: adsorbent characterization, optimization by RSM and CCD, equilibrium and kinetic studies.Environmental science and pollution research international · 2019
- The Bio-Safety Concerns of Three Domestic Temporary Hair Dye Molecules: Fuchsin Basic, Victoria Blue B and Basic Red 2.Molecules (Basel, Switzerland) · 2019
- Aluminum-Crosslinked Nanocellulose Scaffolds for Fluoride Removal.Nanomaterials (Basel, Switzerland) · 2024
- Rapid magnetic solid-phase extraction of Congo Red and Basic Red 2 from aqueous solution by ZIF-8@CoFe2 O4 hybrid composites.Journal of separation science · 2016
via PubMed
Wikidata facts
- Mass
- 350.13
- Image
- Sample of Safranin O.jpg
Show 3 more facts
- chemical formula
- C₂₀H₁₉ClN₄
- canonical SMILES
- CC1=CC2=C(C=C1N)[N+](=C3C=C(C(=CC3=N2)C)N)C4=CC=CC=C4.[Cl-]
- Commons category
- Safranin
Sources (2)
via Wikidata · CC0
~3 min read
Article
2 sectionsContents
- Safranines
- References
Safranin (Safranin O or basic red 2) is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring cell nuclei red. This is the classic counterstain in both Gram stains and endospore staining. It can also be used for the detection of cartilage, mucin and mast cell granules.
Safranin typically has the chemical structure shown at right (sometimes described as dimethyl safranin). There is also trimethyl safranin, which has an added methyl group in the ortho- position (see Arene substitution pattern) of the lower ring. Both compounds behave essentially identically in biological staining applications, and most manufacturers of safranin do not distinguish between the two. Commercial safranin preparations often contain a blend of both types.