Pullulanase
Sign in to saveAlso known as pullulan 6-glucanohydrolase, pullulan alpha-1,6-glucanohydrolase, alpha-dextrin endo-1,6-alpha-glucosidase, amylopectin 6-glucanohydrolase
Pullulanase (, limit dextrinase, amylopectin 6-glucanohydrolase, bacterial debranching enzyme, debranching enzyme, α-dextrin endo-1,6-α-glucosidase, R-enzyme, pullulan α-1,6-glucanohydrolase) is a specific kind of glucanase, an amylolytic exoenzyme, that degrades pullulan. It is produced as an extracellular, cell surface-anchored lipoprotein by Gram-negative bacteria of the genus Klebsiella. Type I pullulanases specifically attack α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages. It is also produced by some other bacteria and archaea. Pullulanase is used as
Key facts
- Enzyme.Name
- Pullulanase
- Enzyme.EC_number
- 3.2.1.41
- Enzyme.CAS_number
- 9075-68-7
- Enzyme.image
- 2yoc.jpg
- Enzyme.width
- 270
- Enzyme.caption
- Debranching enzyme homotetramer, Klebsiella oxytoca
via Wikipedia infobox
Wikidata facts
Show 1 more fact
- EC enzyme number
- 3.2.1.41
Sources (2)
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Article
3 sectionsContents
- Pullulanase enzyme in the food industry
- References
- External links
Pullulanase (, limit dextrinase, amylopectin 6-glucanohydrolase, bacterial debranching enzyme, debranching enzyme, α-dextrin endo-1,6-α-glucosidase, R-enzyme, pullulan α-1,6-glucanohydrolase) is a specific kind of glucanase, an amylolytic exoenzyme, that degrades pullulan. It is produced as an extracellular, cell surface-anchored lipoprotein by Gram-negative bacteria of the genus Klebsiella. Type I pullulanases specifically attack α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages. It is also produced by some other bacteria and archaea. Pullulanase is used as a processing aid in grain processing biotechnology (production of ethanol and sweeteners).
Pullulanase is also known as pullulan-6-glucanohydrolase (Debranching enzyme). Its substrate, pullulan, is regarded as a chain of maltotriose units linked by α-1,6-glycosidic bonds. Pullulanase will hydrolytically cleave pullulan (α-glucan polysaccharides).