5'-nucleotidase ecto
Sign in to saveAlso known as 5'-NT, ecto-5'-nucleotidase, NT5E, Purine 5-Prime-Nucleotidase, 5'-nucleotidase
5′-nucleotidase (5′-NT), also known as ecto-5′-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine.
Protein · UniProt
5'-nucleotidase
- Gene
- NT5E
- Organism
- Homo sapiens (Human)
- Length
- 574 aa
- Molecular mass
- 63,368 Da
- Evidence
- 1: Evidence at protein level
Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084)
Swiss-Prot (reviewed) · via UniProt
Research
1,608 papers- Targeting ecto-5'-nucleotidase: A comprehensive review into small molecule inhibitors and expression modulators.European journal of medicinal chemistry · 2023
- The elegant complexity of mammalian ecto-5'-nucleotidase (CD73).Trends in cell biology · 2021
- Role of ecto-5'-nucleotidase in bladder function.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024
- Ectonucleoside triphosphate diphosphohydrolases and ecto-5'-nucleotidase in purinergic signaling: how the field developed and where we are now.Purinergic signalling · 2021
- Ecto-5'-nucleotidase (CD73): an emerging role as prognostic factor in allergic sensitization.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2024
via PubMed
Clinical Trials
2 registered- PHASE2COMPLETEDPhase II Study of Ribose, Uridine, and Thymidine for a Complex Syndrome Involving Excessive 5'-Nucleotidase ActivityNational Center for Research Resources (NCRR) · NCT00004658
- NACOMPLETEDEffect of Oral Zinc Sulfate Supplementation on Enzymes of Urate PathwayBabylon University · NCT03361618
Wikidata facts
- Instance of
- protein
Show 2 more facts
- cell component
- plasma membrane
- found in taxon
- Homo sapiens
Sources (3)
via Wikidata · CC0
~6 min read
Encyclopedic overview
13 sectionsContents
- Function
- Immunosuppression
- Transcription factor binding sites
- Clinical significance
- Calcification of joints and arteries
- Systemic lupus erythematosus
- Cancer
- Anti-tumor immune response
- As a drug target
- See also
- References
- Further reading
- External links
5′-nucleotidase (5′-NT), also known as ecto-5′-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine.
== Function == Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g. see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.
Excerpted from Wikipedia’s “5'-nucleotidase ecto” article, available under the CC BY-SA 4.0 licence.